cdc biofilm reactor models  (BioSurface Technologies Corporation)

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Phage-antibiotic combinations against multidrug-resistant Pseudomonas aeruginosa in in vitro static and dynamic biofilm models

doi: 10.1128/aac.00578-23

Figure Lengend Snippet: Biofilm time-kill analyses for AR351. Biofilm time-kill analyses of CIP-COL, CIP-AZT, CIP-AMK, and CIP-MEM alone and in combination with EM-T3762626-2_AH (EM) phage against AR351 at 0.25× MIC (COL 0.25 µg/mL, AZT 16 µg/mL, AMK 16 µg/mL, CIP 0.25 µg/mL, and MEM 8 µg/mL) and a theoretical MOI of 1.0. Subinhibitory antibiotic concentrations are used to mimic the expected drug ineffectiveness that would otherwise not occur in this simplified in vitro system. The bars represent the difference in 24-h CFU/cm2 from baseline. (A) Strain AR351 versus ciprofloxacin (1/4 MIC) and/or meropenem (1/4 MIC) with and without phage EM (MOI 1.0). (B) Strain AR351 versus ciprofloxacin (1/4 MIC) and/or colistin (1/4 MIC) with and without phage EM (MOI 1.0). (C) Strain AR351 versus ciprofloxacin (1/4 MIC) and/ or aztreonam (1/4 MIC) with and without phage EM (MOI 1.0). (D) Strain RAR351 versus ciprofloxacin (1/4 MIC) and/or amikacin (1/4 MIC) with and without phage EM (MOI 1.0). Values are means ± standard deviations from two biological replicates. *Regimen(s) that demonstrated bactericidal activity. **Regimen(s) that demonstrated synergy. ***Regimen(s) that demonstrated both synergy and bactericidal activity. AMK, amikacin; AZT, aztreonam; CIP, ciprofloxacin; COL, colistin; EM, phage EM-T3762627-2_AH; MEM, meropenem; MIC, minimum inhibitory concentration; MOI, multiplicity of infection.

Article Snippet: The in vitro model consisted of a previously described CDC biofilm reactor (CBR) model (BioSurface Technologies, Bozeman, MT, USA) set up with polyurethane coupons inserted into eight rods, simulating human PK, to evaluate the in vitro activity of antimicrobials and phage ( 32 ).

Techniques: In Vitro, Activity Assay, Concentration Assay, Infection

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Phage-antibiotic combinations against multidrug-resistant Pseudomonas aeruginosa in in vitro static and dynamic biofilm models

doi: 10.1128/aac.00578-23

Figure Lengend Snippet: Biofilm time-kill analyses for I0003-1. Biofilm time-kill analyses of CIP-COL, CIP-AZT, CIP-AMK, and CIP-MEM alone and in combination with 14207 phage against RI0003-1 at 0.25× MIC (COL 0.25 µg/mL, AMK 4 µg/mL, AZT 16 µg/mL, CIP 0.125 µg/mL, and MEM 4 µg/mL) and a theoretical MOI of 1.0. Subinhibitory antibiotic concentrations are used to mimic the expected drug ineffectiveness that would otherwise not occur in this simplified in vitro system. The bars represent the difference in 24-h CFU/cm2 from baseline. (A) Strain RI0003-1 versus ciprofloxacin (1/4 MIC) and/or meropenem (1/4 MIC) with and without phage 14207 (MOI 1.0). (B) Strain RI0003-1 versus ciprofloxacin (1/4 MIC) and/or colistin (1/4 MIC) with and without phage 14207 (MOI 1.0). (C) Strain RI0003-1 versus ciprofloxacin (1/4 MIC) and/ or aztreonam (1/4 MIC) with and without phage 14207 (MOI 1.0). (D) Strain RI0003-1 versus ciprofloxacin (1/4 MIC) and/or amikacin (1/4 MIC) with and without phage 14207 (MOI 1.0). Values are means ± standard deviations from two biological replicates. *Regimen(s) that demonstrated bactericidal activity. **Regimen(s) that demonstrated synergy. ***Regimen(s) that demonstrated both synergy and bactericidal activity. 14207, 14207 phage; AMK, amikacin; AZT, aztreonam; CIP, ciprofloxacin; COL, colistin; MEM, meropenem; MIC, minimum inhibitory concentration; MOI, multiplicity of infection.

Article Snippet: The in vitro model consisted of a previously described CDC biofilm reactor (CBR) model (BioSurface Technologies, Bozeman, MT, USA) set up with polyurethane coupons inserted into eight rods, simulating human PK, to evaluate the in vitro activity of antimicrobials and phage ( 32 ).

Techniques: In Vitro, Activity Assay, Concentration Assay, Infection

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Phage-antibiotic combinations against multidrug-resistant Pseudomonas aeruginosa in in vitro static and dynamic biofilm models

doi: 10.1128/aac.00578-23

Figure Lengend Snippet: Comparison of five antimicrobial classes plus phage (EM or 14207) against I0003-1 and AR351. Average biofilm cell reduction seen with single phage plus antibiotic combinations against AR351 and I0003-1 in biofilm time-kill analyses of EM or 14207, respectively, with CIP, MEM, AZT, AMK, and COL at 0.25× MIC and a theoretical MOI of 1.0. Subinhibitory antibiotic concentrations are used to mimic the expected drug ineffectiveness that would otherwise not occur in this simplified in vitro system. The bars represent the difference in 24-h CFU/cm2 from baseline. Values are means ± standard deviations from two biological replicates. 14207, 14207 phage; AMK, amikacin; AZT, aztreonam; CIP, ciprofloxacin; COL, colistin; EM, EM phage; MEM, meropenem; MIC, minimum inhibitory concentration; MOI, multiplicity of infection; TK, time-kill.

Article Snippet: The in vitro model consisted of a previously described CDC biofilm reactor (CBR) model (BioSurface Technologies, Bozeman, MT, USA) set up with polyurethane coupons inserted into eight rods, simulating human PK, to evaluate the in vitro activity of antimicrobials and phage ( 32 ).

Techniques: Comparison, In Vitro, Concentration Assay, Infection

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Phage-antibiotic combinations against multidrug-resistant Pseudomonas aeruginosa in in vitro static and dynamic biofilm models

doi: 10.1128/aac.00578-23

Figure Lengend Snippet: Biofilm model for AR351 versus phage cocktail ± ciprofloxacin. In vitro PK/PD biofilm model results for all phage and CIP combinations against P. aeruginosa strain AR351. The error bars indicate standard deviation between repeats. *Regimens that met their definition for improvement. **Regimens that met their definition for enhancement.

Article Snippet: The in vitro model consisted of a previously described CDC biofilm reactor (CBR) model (BioSurface Technologies, Bozeman, MT, USA) set up with polyurethane coupons inserted into eight rods, simulating human PK, to evaluate the in vitro activity of antimicrobials and phage ( 32 ).

Techniques: In Vitro, Standard Deviation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Phage-antibiotic combinations against multidrug-resistant Pseudomonas aeruginosa in in vitro static and dynamic biofilm models

doi: 10.1128/aac.00578-23

Figure Lengend Snippet: Resistance evaluations for strains AR351 and I0003-1 a

Article Snippet: The in vitro model consisted of a previously described CDC biofilm reactor (CBR) model (BioSurface Technologies, Bozeman, MT, USA) set up with polyurethane coupons inserted into eight rods, simulating human PK, to evaluate the in vitro activity of antimicrobials and phage ( 32 ).

Techniques:

Journal: Lasers in surgery and medicine

Article Title: Antimicrobial blue light (405-nm) inactivation of microbial isolates in biofilms

doi: 10.1002/lsm.23159

Figure Lengend Snippet: Cell density of 24- or 48-h old biofilms in 96-well microtiter plates.

Article Snippet: Biofilms of A. baumannii AF0005, P. aeruginosa AF0001, and IQ0042, some of the most sensitive strains to 405-nm aBL in the microplates, were also developed under dynamic shear conditions using the CDC biofilm reactor (model CBR 90–1 DH, BioSurface Technologies Corporation, USA) as described elsewhere 30 , 31 .

Techniques:

Journal: Lasers in surgery and medicine

Article Title: Antimicrobial blue light (405-nm) inactivation of microbial isolates in biofilms

doi: 10.1002/lsm.23159

Figure Lengend Snippet: Irradiance 60 mW/cm2, irradiation time was 60 min for all the strains except for N. gonorrhoeae 179 that was irradiated for 30 min (total radiant exposure of 216 or 108 J/cm2, respectively). ns: not significant, ** P<0.01, **** P<0.0001. The differences between untreated or treated biofilms were analyzed with a one-way ANOVA followed by Tukey’s multiple comparison test. Data represented are mean ± standard error. All experiments were performed in triplicate.

Article Snippet: Biofilms of A. baumannii AF0005, P. aeruginosa AF0001, and IQ0042, some of the most sensitive strains to 405-nm aBL in the microplates, were also developed under dynamic shear conditions using the CDC biofilm reactor (model CBR 90–1 DH, BioSurface Technologies Corporation, USA) as described elsewhere 30 , 31 .

Techniques: Irradiation, Comparison

Journal: Lasers in surgery and medicine

Article Title: Antimicrobial blue light (405-nm) inactivation of microbial isolates in biofilms

doi: 10.1002/lsm.23159

Figure Lengend Snippet: Irradiance 60 mW/cm2, irradiation time was 60 min for all the strains except for N. gonorrhoeae 179 that was irradiated for 30 min (total radiant exposure of 216 or 108 J/cm2, respectively). ns: not significant, ** P<0.01, **** P<0.0001. The differences between untreated or treated biofilms were analyzed with a one-way ANOVA followed by Tukey’s multiple comparison test. Data represented are mean ± standard error. All experiments were performed in triplicate.

Article Snippet: Biofilms of A. baumannii AF0005, P. aeruginosa AF0001, and IQ0042, some of the most sensitive strains to 405-nm aBL in the microplates, were also developed under dynamic shear conditions using the CDC biofilm reactor (model CBR 90–1 DH, BioSurface Technologies Corporation, USA) as described elsewhere 30 , 31 .

Techniques: Irradiation, Comparison

Journal: Lasers in surgery and medicine

Article Title: Antimicrobial blue light (405-nm) inactivation of microbial isolates in biofilms

doi: 10.1002/lsm.23159

Figure Lengend Snippet: Cell density of 48-h old biofilms in the CDC biofilm reactor.

Article Snippet: Biofilms of A. baumannii AF0005, P. aeruginosa AF0001, and IQ0042, some of the most sensitive strains to 405-nm aBL in the microplates, were also developed under dynamic shear conditions using the CDC biofilm reactor (model CBR 90–1 DH, BioSurface Technologies Corporation, USA) as described elsewhere 30 , 31 .

Techniques:

Journal: Lasers in surgery and medicine

Article Title: Antimicrobial blue light (405-nm) inactivation of microbial isolates in biofilms

doi: 10.1002/lsm.23159

Figure Lengend Snippet: Irradiance 60 mW/cm2, irradiation time was 60 min (total radiant exposure of 216 J/cm2). ns: not significant. The differences between untreated or treated biofilms were analyzed with a one-way ANOVA followed by Tukey’s multiple comparison test. Data represented are mean ± standard error. All experiments were performed at least in two independent experiments in duplicate coupons.

Article Snippet: Biofilms of A. baumannii AF0005, P. aeruginosa AF0001, and IQ0042, some of the most sensitive strains to 405-nm aBL in the microplates, were also developed under dynamic shear conditions using the CDC biofilm reactor (model CBR 90–1 DH, BioSurface Technologies Corporation, USA) as described elsewhere 30 , 31 .

Techniques: Irradiation, Comparison